Spectroscopy Lab Report

Thursday, October 21, 2021 6:45:46 AM

Spectroscopy Lab Report



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Atomic Absorption Spectroscopy Lab

Bradford for protein determination was used in the lab in order to determine the concentration of the unknown 2 and the previously purified protein. This protein dye complex is the reason for a shift in the dye absorption with maximum between to nm. This derived amount of absorption does not require dilution and can be used for both microassays and standard solutions. The reagent used was the combination of Bradford Reagent-Brilliant Blue G in phosphoric acid and methanol.

This solution was moved into the crystal container for UV spectroscopy. The UV spectra was taken from to nm and the concentration of the sample was 2. The absorbance was recorded at nm and nm. Results The carvone product was weighed to be 1. The starting mass of spearmint oil was 2. As and when a positive finding of DNA is obtained, it was quantified using a UV Spectrophotometer and its purity was checked for each sample to be used in Polymerase chain reaction based amplification.

DNA quantification and quality assessment using UV spectrophotometry. Essays Essays FlashCards. Browse Essays. Sign in. Essay Sample Check Writing Quality. Show More. Read More. Unlike the chromatographies, which physically separate materials, infrared. It is optimized for high flux and good energy resolution. Multi-user software runs on the company intranet and provides up to. Establish spacing of optical spectrometer diffraction grating.

Part 1, which also appears in your lab book, follows next. General Chemistry Laboratory. Esl best essay ghostwriters for hire australia. Spectroscopy lab report By write my essay today Help me write philosophy papers 30 March, assignment helper online , friction lab report. Spectroscopy lab report. Additionally, while changing the receiver from cyclohexane to toluene there was a loss of distillate which also led to the errors observed in the data. Furthermore, if more data were collected for each compound it would be a better representation of the experiment's results. If these errors were avoided, then the experiment would be more efficient in distilling the two compounds from each other and the plateau would be as sharp as figure 6 in the lab.

This also highlights the fact that diastereomers have different chemical properties, such as melting point. Since the experimental melting point range almost overlaps with the expected range, there is little error in this measurement. The small amount of error could have occurred from the MelTemp temperature rising too quickly, leading to errors when reading the. The melting points for the acidic and neutral compounds were hence too low, and the melting point for the basic compound was too high. The errors in the acidic and neutral compounds can be explained by impurities in the crude product, along with the presence of the solvents, ethyl acetate and water, which disturbed the stability of the compounds, and led to lower melting points over a larger range.

The abnormally high melting point of the base may have been observed due to improper separation of the acid and base solutions in the separation funnel. Some of the acidic compound may have entered the basic solution and reacted with the base to form a high melting point salt, making the melting point of the base appear abnormally high. The Mel-Temp was also turned on a high setting accidentally, so it is possible that the temperature rose too quickly to get a good reading of the melting. Light intensity is one of factors affecting photosynthesis.

Depending on distance of light intensity, the result would be changed. Prior to the experiment, the distance should be settled. Even though it was expressed by using tape, it would be inexact. Therefore, improvement to the method would be to make the higher light intensities so as to be quite accurate, which denotes lamps would be adjusted to a beaker containing elodea from side to side because the smaller light intensities would be less accurate since the light spreads out, and the elodea might be exerted influence on other experiments or background light. They have a relatively high level of hydrophilicity. Conversely, cationic surfactants such as cetyltrimethyl ammonium bromide CTAB release a positive charge in an aqueous solution.

On the other hand, in alkali pH, amphoteric surfactants act as anionic while in acid pH, it act as cationic. Non-ionic surfactants such as partially hydrolyzed PVA polyvinyl alcohol have no charge Li et al, Among four types of surfactants, partially hydrolyzed PVA is most frequent used because it gives the smallest microsheres Jeffrey et al, The isolated degradation products are subjected to Mass studies to obtain their accurate mass fragment patterns. On the basis of results complete degradation path for AN has been established. We have also. Even though cooling crystallization and anti-solvent crystallization are convenient, it has major drawbacks in terms of slow rate of crystallization, high supersaturation degree [10], solvent and anti-solvent selection based on solubility[11].

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