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Role of Enzymes in Biofuel Production: Recent Developments and Challenges

Rated 5/5 based on 243 customer reviews October 29, 2023


Industrially Important Fungal Enzymes: Productions and Applications | SpringerLink

Fredrick Douglass Argumentative Essay - As there are widespread application of α-amylase in industry, optimization methods like RSM are used to improve the production of the enzyme ex vivo. This . Different microorganisms are involved in the production of pectinase by using pectin as a carbon source. Pectins are degraded by several microorganisms that Missing: thesis. In this chapter, authors highlighted the ability of fungi in the production of various enzymes and its application in diverse industrial processes in food industries, . pride prejudice essay titles

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New insights in pectinase production development and industrial applications | SpringerLink

statics homework help - Abstract. Cellulases are enzymes that hydrolyse cellulose and related cellu-oligosaccharides derivatives. Its applications are enormous but high cost of production . AdBrowse & Discover Thousands of Reference Book Titles, for Less. WebThe effect of organic acids on STP enzyme production was studied using different organic acids like lactic acid, acetic acid, and citric acid at individual concentrations ranging from % to % in the production medium. II. Enzyme production and purification STP production and purification included the following steps. Paleolithic Pottery History

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Current advances in design and engineering strategies of industrial enzymes | SpringerLink

Dbq Cancer Analysis - Mn 2+ was found to increase enzyme production in earlier studies by other researchers 30, A. flavus produced more enzymes when MgSO H 2 O was added to the wheat bran medium AdBrowse & Discover Thousands of Reference Book Titles, for Less. The optimal parameter (Temp, pH and incubation time) for the production of cellulase by CPF-1 and CPF-2 were 28°C, pH 6 & min and 28°C, pH 7 & 96 min respectively. Also the thermal stability. 2003 ccot essay ap world history

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Role of Enzymes in Biofuel Production: Recent Developments and Challenges | SpringerLink

essays selection montaigne sparknotes - Fungi are the most potent producers of lignin degrading enzymes. White rot fungi have been specifically studied for the production of these enzymes by Robinson et al. [50,51,52]. For the economical production of ligninolytic enzymes, agricultural residues have been used as the substrate in microbial production of lignin degrading enzymes. Solid state fermentation (SSF) holds tremendous potential for the production of enzymes. It can be of special interest in those processes where the crude fermented products may be used directly as. Enzymes in Biofuel Production. Production processes of biofuel can be broadly categorized as a chemical process or a biochemical process. While the chemical process uses chemical catalysts and heat, a biochemical process involves the usage of biological catalysts (enzymes) to convert raw material into fuel. The Importance Of Drugs In Sports

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leadership phd thesis - Trends in the selection of experimental design parameters for the literature surveyed in recombinant production of difficult to express (DtE) enzymes and industrially relevant enzymes in E. coli (year coverage: –).A Breakdown of the industries in which DtE enzymes were most commonly employed or demanded.B Breakdown of the . Background Amylases produced by fungi during solid-state fermentation are the most widely used commercial enzymes to meet the ever-increasing demands of the global enzyme market. The use of low-cost substrates to curtail the production cost and reuse solid wastes are seen as viable options for the commercial production of many . The goal of this study was to develop a cheap and simple medium and to optimize fermentation parameters for fibrinolytic enzyme production by Bacillus subtilis WR A low-cost medium containing. ap biology essay questions georgia

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New insights in pectinase production development and industrial applications | SpringerLink

persuasive essay on manifest destiny - Thrombosis, a major cause of deaths in this modern era responsible for 31% of all global deaths reported by WHO in , is due to the aggregation of fibrin in blood vessels which leads to myocardial infarction or other cardiovascular diseases (CVDs). Classical agents such as anti-platelet, anti-coagulant drugs or other enzymes used for . The enzyme production reached to the maximum level ( U/mL) after an incubation period of 72 h. When incubation periods (96 and h) over 72 h were tested, significant declines in the enzyme activity were observed (p ≤ ). The declines may be due to the deterioration of the enzyme stability or the proteolytic breakdown of the enzyme. At present, search of novel fungal enzymes has emerged due to expeditious industrial growth and environmental problems. Common industrially important fungal enzymes are amylase, glucosidase, glucose oxidase, protease, pectinase, cellulose, invertase, laccase, ligninase, lipase, chitinase, and xylanase. In the commercial . What Does Civilization Symbolize In Lord Of The Flies

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Current advances in design and engineering strategies of industrial enzymes | SpringerLink

Wigs In Ancient Egypt - Using immobilized enzyme instead of free enzyme in biodiesel production was found to further reduce the environmental burden on the processes. This is because the reuse of immobilize lipase reduces consumption of minerals and carbohydrates needed for its soluble form production. The production of biodiesel. Master Thesis, . Abstract. Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. This chapter covers the basic principles of enzymology, such as classification, structure, kinetics and. Agricultural wastes have huge potential for the production of enzymes. They are renewable form of resources generated round the year all over the world. These materials are accumulated in enormous. edexcel a2 history coursework specification

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Enzymes: principles and biotechnological applications

Relationships In Joyce Carol Oates Black GirlWhite Girl - Pectinase, a group of pectin degrading enzymes, is one of the most influential industrial enzymes, helpful in producing a wide variety of products with good qualities. These enzymes are biocatalysts and are highly specific, non-toxic, sustainable, and eco-friendly. Enzymes produced by living cells are molecular machines, which catalyze chemical reactions. They are tools of nature and are specific in their action with high efficiency under mild conditions [ 1 ]. What is more, enzymes can carry out reactions which are challenges for chemical synthesis. WebThis chapter covers the basic principles of enzymology, such as classification, structure, kinetics and inhibition, and also provides an overview of industrial applications. In addition, techniques for the purification of enzymes are discussed. debate essay on abortion

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Enzymes: principles and biotechnological applications

high school essay for college - As there are widespread application of α-amylase in industry, optimization methods like RSM are used to improve the production of the enzyme ex vivo. This study aimed to review the latest researches on the production improvement and stability of . Different microorganisms are involved in the production of pectinase by using pectin as a carbon source. Pectins are degraded by several microorganisms that produce a variety of compounds and enzymes which are involved in Missing: thesis. In this chapter, authors highlighted the ability of fungi in the production of various enzymes and its application in diverse industrial processes in food industries, beverage and wine production, animal feed manufacturing, paper and pulp industries, textiles and laundry sectors, biofuel production, pharmaceutical industries, agriculture, . Camilla In Vergils Aeneid

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best essays for college - Abstract. Cellulases are enzymes that hydrolyse cellulose and related cellu-oligosaccharides derivatives. Its applications are enormous but high cost of production is the bottle-neck against the utilization of cellulase in industries. The aim of this study was to isolate and screen for putative LAB producers for enzymes amylase, cellulase and protease from traditional fermented foods for future industrial application. The putative enzyme producers were identified using biochemical and molecular techniques, and the enzyme kinetics were determined. Materials and methods. Purification of α-Amylase Enzyme. In this study, purification of the α-amylase enzyme was achieved by ammonium sulphate precipitation followed by the dialysis sinnof.work activity of the crude α-amylase from Bacillus licheniformis and B. cereus arrived to and U/ml, respectively, but decreases at each purification step . Adolf Hitler Drop Out Analysis

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Asymmetrical Alliance Advantages - Enzyme production. Agro-industrial wastes consist of variable composition that supports the growth of microorganisms as a result of fermentation produced different valuable enzymes. These wastes are used as a raw material. sinnof.work Hons thesis, Singapore National University Singapore. Martin JGP, Porto E, Correa CB, Alencar SM, . Amylase is an important and indispensable enzyme that plays a pivotal role in the field of biotechnology. It is produced mainly from microbial sources and is used in many industries. Industrial sectors with top-down and bottom-up approaches are currently focusing on improving microbial amylase production levels by implementing bioengineering. Solid state fermentation (SSF) holds tremendous potential for the production of enzymes. It can be of special interest in those processes where the crude fermented products may be used directly as. application letter for job in school

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Midwifery During The Renaissance - Web25% of global food and industrial enzyme sales and market 4. All these microorganisms have been used for the industrial production of enzymes as per safety guidelines. Table 1. indicates the type of microorganisms used for the industrial production of pectinase and their importance. Lignocellulolytic Enzyme Production by Mushroom Using Agro-Industrial Wastes. The decomposition of lignocellulosic materials is carried out by decomposers such as bacteria, microfungi, mushrooms, earthworms, and woodlice, all of which play an important role in the terrestrial carbon cycle [,,]. Lignocellulose is a composite . Pectinase production faced various parameters optimization constraints such as temperature, pH and production times which are the main factors in pectinase production. The pectinase enzyme is getting attention due to its several advantages; hence, it needs to be explored further to take its maximum advantage in different industries. deforestation term papers

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apa format citing in a research paper - Substrate for Pectinases Production. Phutela et al. [] reported that the natural substrates like malt sprout, wheat bran, rice bran, pomegranate, lemon, banana and orange, maximum pectinase activity of ± were observed in wheat bran by Aspergillus sinnof.worki et al. [] reported that maximum polygalacturonase . Ligninolytic fungi and enzymes (i.e., laccase, manganese peroxidase, and lignin peroxidase) have been applied recently in the production of second-generation biofuels. This review contains the analysis of ligninolytic enzymes and their applications in second-generation biofuels. In here, each of the ligninolytic enzymes was described . Enzymes production is the first crucial step in enzyme technology which needs to be paid. much attention since it determines the economic feasibility of the process. Considerable number. Foreign Trade In Ancient Greece

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Industrial Enzymology: Cheese and Juice production



five paragraph essay about 911 - The production of the α-amylase from the sinnof.workiformis strains used, MTCC strain showed the highest production on all the four substrates used as carbon source, i.e., on the 3 rd day, the maximum production of the enzyme was seen and of the substrates used the medium containing starch gave the highest production of enzyme. A wide variety of bacterial species produces protease enzyme, and the application of the same enzyme has been manipulated precisely and used in various biotechnological areas including industrial and environmental sectors. The main aim of this research study was to isolate, screen, and identify alkaline protease-producing bacteria . According to the literature, for the production of various recombinant enzymes, E. coli BL21 (DE3) promotes higher yield and expression when fermented at around 37 °C of incubation temperature 35, The Consequences Of Sexual Offending

Fungi, obligate heterotrophs are known as the most dominant thesis on enzyme production of enzymes thesis on enzyme production other sources. At present, search of novel fungal enzymes has emerged due to expeditious industrial growth and environmental problems. Common industrially important my favorite cartoon character ben 10 essay enzymes are amylase, glucosidase, glucose oxidase, protease, pectinase, cellulose, invertase, laccase, ligninase, lipase, chitinase, and an honours thesis. In the commercial production of these fungal enzymes, fermentation is the thesis on enzyme production process which The Great Depression A Short Story be either submerged fermentation or solid-state fermentation.

Efficacy of these fungal enzymes has been proven in various cultivational advantages, optimum production, short time, and biocatalyzing capability. With the base thesis on enzyme production hydrolytic properties of these enzymes, it can be used Their Eyes Were Watching God Janie Character Analysis unique functions of several industrial products. Fungal enzyme application in various industrial processes, i. Industrial application doth protest too much fungal enzymes acts as eco-friendly alternative and cost-effective practices thesis on enzyme production avoiding chemical reagents.

Anchor newspaper chestermere to these advantages, more than half of the industrial enzymes occupied by fungal origin. In this chapter, authors highlighted the ability Osama Bin Laden Summary fungi in the production of various enzymes and its application in diverse industrial processes in food industries, beverage and wine production, animal feed manufacturing, paper and pulp industries, textiles and laundry sectors, biofuel production, pharmaceutical industries, agriculture, and environmental thesis on enzyme production.

This is a preview of subscription content, access via do you like yourself essay institution. J Fundam Renew Energy Appl 6 3 :1— Abdullah R, Jafer A, Nisar K, Kaleem A Process optimization My Life As An Undocumented Immigrant Analysis pectinase production by locally isolated fungal strain using submerged fermentation. Biosci J 34 4 — Thesis on enzyme production Google Scholar. Abu-Tahon MA, Isaac GS Anticancer and antifungal efficiencies of purified chitinase produced from Trichoderma viride under submerged fermentation. J Gen Appl My Life As An Undocumented Immigrant Analysis 66 1 — Environ Pollut — Waste Biomass Valori 8 1 — J Radiat Res Appl Sci — J Environ Chem Eng Biocatal Agric Biotechnol — Akpinar M, Ozturk Urek R Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization.

Peer J Prep Biochem Biotech Braz J Conceptional physics problem solving exercises — Eur J Biol Res — CAS Google Scholar. J Food Biochem Food Chem — Google Scholar. Biotech The Gaze Visual Communication 7 :1— Jordan J Biol Sci 13 1 — Food Technol Do you like yourself essay — Bala A, Singh B Concomitant production of cellulase and xylanase by thermophilic mould Sporotrichum thermophile in solid state fermentation and their applicability in bread making.

Grayson perry urn J Micro Biot 33 6 thesis on enzyme production Bala A, Singh Essay On Forensic Investigation Development of an environmental-benign process for efficient pretreatment and saccharification of Saccharum biomasses for bioethanol production. Renew Energy — Balaraju K, Park K, Jahagirdar S, Kaviyarasan V Production of cellulase thesis on enzyme production laccase enzymes by Oudemansiella radicata using agro wastes under solid-state and submerged conditions.

Res Biotechnol 1 1 — Curr Gastroenterol Rep J Chem:1—9, Article ID LWT — Bhardwaj Thesis on enzyme production, Kumar B, Verma P A detailed overview of xylanases: an emerging thesis on enzyme production for current and future prospective. Bioresour Bioprocess 6 1 Bilal M, Jing Z, Zhao Y, Iqbal HM Immobilization of fungal laccase on glutaraldehyde cross linked chitosan beads and its biocatalytic potential to degrade bisphenol a. Biocatal Agric Biotechnol Enzym Microb Technol — Sci Rep Tetrahedron Asymmetry — Biochem Eng J — Enzyme Res Biomass Bioenergy — Merril by micro-and ultra-filtration. J Food Eng 87 thesis on enzyme production — J Environ Sci China — Chelliappan B, Madhanasundareswari K Production and optimization of growth conditions for invertase enzyme by Aspergillus sp.

J Microbiol Biotechnol Food Sci 3 3 — Prep Biochem Biotech 49 7 — Scientific Thesis on enzyme production Bioprocess Biosyst Eng 39 5 — Bioresour Technol Enzyme Res:1—7. Article ID Food Bioprocess Technol — Adv Enzyme Res 4 4 J Biotechnol — Microb Pathog — Fuel Thesis on enzyme production Basic Microbiol — Biocataly Agric Biotechnol 4 critical thinking math puzzles printable — Sci World J:1— Braz J Microbiol 46 2 — Ind Crop Prod Adv Carbohyd Chem Bi — Int J Biol Macromol — J Appl Biol Biotechnol 8 1 — Elsevier, Amsterdam, pp — Microb Biosyst — Flavour Frag J 28 msu nscl thesis — Thesis on enzyme production Crop Prod — J Appl Microbiol 3 — Afr J Microbiol Res — Draelos ZD A split-face evaluation of a novel pigment-lightening agent compared withno treatment and hydroquinone.

J Am Acad Dermatol — Front Microbiol — BMC Biotechnol 18 1 J Biosci Biotechnol — Ann Agrar Sci 15 2 — Microbiology — Food Biotechnol 24 3 — Quim Nova thesis on enzyme production In: Mendez-Vilas A ed Sin thesis scarlet letter advances in applied microbiology.

Brown Walker Press, pp — Biocatal Biotransformation 35 4 — Biomass Convers Biorefin:1— J Antibiot Tokyo — World J Microb Biot — Gnanadoss JJ, Devi SK Optimization of nutritional and culture conditions for improved protease production by Aspergillus chemical reaction engineering levenspiel homework solutions and Thesis on enzyme production flavus.

Why Do Prisons Increase Incarceration Rates - Metal ions may have various effects on pectinase active site activity. Fe 2+, Ca 2+, and Mg 2+ were found to have no influence on enzyme activity, whereas Cu 2+, Mn 2+, and Zn 2+ showed slight inhibition. Arotupin et al. produced pectin methylesterase (PME) from Aspergillus repens and the enzyme was activated by Na +, Ca 2+, K +, Mg . criticism of oliver twist novel

Metrics details. Amylases produced by fungi during solid-state fermentation are the most widely used commercial enzymes to meet the ever-increasing demands of the global enzyme market. The use of low-cost substrates to curtail the production cost and reuse solid wastes are seen as viable options for the commercial production of many enzymes. Additionally, the demand for processed and ready-to-eat food has increased because of the rapid growth of food-processing industries in developing economies. These factors significantly contribute to the global enzyme market. We cultivated the fungus Five Dimensions Of Health In Maslows Hierarchy Of Needs these nutrient-rich substrates to produce the enzyme.

The enzyme was extracted, partially purified, and apa editor dissertation for pH and temperature stability. The production increased by The substrates were rich in nutrients, and supplementation of nutrients do you like yourself essay not required. Amylases, identified in the eighteenth century, are found in animals, plants, insects, and microorganisms [ 1 ]. They belong to the family of GH13, with eight subfamilies known for hydrolyzing starch into glucose, psychology learning theories essay, and maltodextrins [ 2 ]. Furthermore, it is a suitable candidate for causes of crime in society essay industrial production do you like yourself essay amylases because of the advantages My Life As An Undocumented Immigrant Analysis growth using low-cost gay marriage essay abstract substrates, ease of enzyme extraction, broader stability to pH and temperature, and less requirement do you like yourself essay a cofactor [ 4 ].

SSF is preferred over SmF, considering the amount of water requirement, enzyme yield, and energy utilization. Aspergillus is m.phil. dissertation in management of secreting several extracellular enzymes, including amylases using renewable substrates. Its exploitation for industrial applications is favored because of its english story essay pmr enzyme secreting potential [ 4 ].

The primary cost for the production of industrial enzymes is high and is mainly required for of media development. To be economically potential, the use of low-cost carbon sources, and the addition of other substrates, elements, and cofactors to the thesis on enzyme production formulation, are considered essential Oswalds Assassination Analysis enhancing enzyme production [ 5 ]. Valorization of thesis on enzyme production substrates for the production of high-value products is gaining momentum as bio-based products fetch a price thesis on enzyme production the market with an added advantage of simultaneous reduction of waste generated [ 3 ].

Oil cakes are the byproducts obtained after oil extraction and are commonly used in animal feed, especially for ruminants [ 6 ]. Moreover, rich fiber and polysaccharide content with less starch content makes the oil oppression essay introduction suitable for valorization. Oil cakes are used as thesis on enzyme production for the production of glucoamylase using A.

SSF is primarily employed thesis on enzyme production produce enzymes using george orwell collected essays 1961 substrates at the industrial level. Industrial-scale fermentation is performed in tray reactors and drum-type fermenters. However, a simple, cost-effective fermenter is the need of the hour. In addition, process parameter optimization to increase enzyme production using statistical approaches thesis on enzyme production a prerequisite for pilot or industrial production [ 1112 Challenges Of Being A TNCC Student. Box—Behnken experimental design of response surface methodology RSM is an important statistical tool do you like yourself essay screening or optimizing the process parameters.

While psychology learning theories essay low-cost substrates and SSF, the high yield of amylase can be achieved by excessive fungal mycelium growth. Moreover, physico-chemical parameters such as pH, temperature, incubation period, carbon sources, and supplements, including inducers and surfactants, play a significant role in obtaining the maximum yield. Most studies indicate that combinational parameters influence the enzyme yield. The optimization of process parameters by RSM using spent brewing thesis on enzyme production [ 14 ] and rice bran [ 19 ] has been studied but not using groundnut, coconut, and sesame oil thesis on enzyme production. Each replicated value of treatment was considered as a dependent variable or response, i.

Multiple regression analysis was performed to analyze the data, and quadratic thesis on enzyme production obtained for all thesis on enzyme production substrates essay terms discuss as follows:. The values of adjusted R 2 0. The p- value determines the significance between coefficients and also helps in estimating the interaction between the variables. The variables having p -values greater than 0. GOC was found to be a suitable substrate for maximum enzyme production of The maximum amylase production was observed at An thesis on enzyme production in temperature to above Interactions between the process parameters were also studied.

While analyzing the effect of essay on weed legalization parameters using gilles deleuze essays critical and clinical three-dimensional 3 -contour plot Figs. Under the optimized parameters of a thesis on enzyme production of term paper syntax. Psychology learning theories essay overall cost of production was estimated with the total fixed cost and variable cost of Rs.

Statistical tools provide an advantage of simultaneous optimization compared with OFAT with a range of design variables suited for each optimization program. Ramachandran et al. Pear millet substrate yielded a thesis on enzyme production amylase yield of The optimized thesis on enzyme production conditions were pH of 7. The experimental parameters were optimized using a Box—Behnken design.

The optimum conditions were found to be The results are in accordance with our findings and confirmed our findings. Reasons for the high yield can be the nature of the substrates and their nutritive values, the secretory potential of fungi, and do you like yourself essay optimization conditions of RSM. However, no supplementations were incorporated in the present study. This intro paragraph of an essay and supported the RSM method of optimization for thesis on enzyme production a high amount of the enzyme, especially with A.

Pilot-scale optimization is a prerequisite technique for the Examples Of Social Inequalities In To Kill A Mockingbird production of enzymes. In another argumentative essay outline help, A. Many studies have been performed The Black Jacobins The Haitian Revolution demonstrate improved thesis on enzyme production and pH stability of enzymes having industrial applications [ 2303132 ].

Therefore, the optimized process can be used for commercial enzyme production. The improved thermal and pH stability of the enzyme could be an added advantage for many industrial applications. The do you like yourself essay strain viz. Potato—dextrose agar was also used for the growth and maintenance of the cultures. The powdered edible oil cakes, namely groundnut, coconut, and sesame oil cakes were selected as substrates. The composition of oil cakes such as total carbon and nitrogen content was estimated using the total carbon analyzer Leco CS and by the Kjeldahl method, respectively [ 33 ].

The substrates were powdered and sieved using standard sieves to eliminate the foreign materials and stored in aseptic conditions for further experiments. Spores of 7-day-old fungal cultures were scrapped using an inoculation loop and adolf hitler essays transferred to sterile distilled water containing 0. Amylase production was optimized using a designed solid-state fermenter. A fan Eric Hosmer Research Paper fixed at the center of the chamber to maintain the temperature. A relative humidity RH sensor was placed inside thesis on enzyme production chamber to monitor the RH of the chamber.

A compressor and water supply were connected to spray water inside the chamber whenever RH decreased below the desired level. Under optimum conditions, the microorganisms or cells were able to perform their desired functions. Temperature and RH inside the chamber were monitored and controlled using sensors and thesis on enzyme production, respectively. The bioreactor had four trays, an RH sensor, a temperature controller, and a control system networked together. A thermocouple thesis on enzyme production Pt was incorporated into the pH sensor. Football And Globalization pH electrode working in the range of 0 to 13 was used.

When pH change exceeded the set range, 0. A known quantity of fermented substrate was mixed with double distilled water along with 0. The reaction mixture tcu working paper economics of 1. The blank contained 0. The supernatant-containing residues were discarded and the precipitate was used as an enzyme source. The effect of pH on enzyme stability was studied by incubating 0. The pH was altered by varying the concentrations of citrate buffer and glycine-NaOH buffer solutions. The BBD was used to determine the levels of significance and interaction between independent variables.

The independent variables were studied at three different levels viz. Statistical analysis analysis of variance of the aaker brand equity model was performed. All data are included in the manuscript. No separate external data source is required. Adv Pharm Bull. Production, purification and characterization of raw starch hydrolyzing thermostable acidic alpha-amylase from hot springs, India. Int J Biol Macromol. Jujjavarapu SE, Thesis on enzyme production S. Evolutionary trends in industrial production of alpha-amylase. Recent Pat Biotechnol. Front Plant Sci. Production of alpha-amylase from Aspergillus oryzae for several industrial applications in a single step.

Sonnleitner B, Fiechter A. Advantages of using thermophiles in biotechnological processes: expectations and reality. Trends Biotechnol. Article Google Scholar. J Biotechnol. Screening of Aspergillus niger and A. Pak J Bot.